Nanosensor detection of reactive oxygen and nitrogen species leakage in frustrated phagocytosis of nanofibres

Yu-Ting Qi (1) , Fu-Li Zhang (1) , Si-Yu Tian (1) , Hui-Qian Wu , Yi Zhao (2) , Xin-Wei Zhang (1) , Yan-Ling Liu (1) , Pingqing Fu (3) , Christian Amatore (4) , Wei-Hua Huang (1)

1 Wuhan University [China]
2 Indiana University [Bloomington]
3 Institute of Surface-Earth System Science of Tianjin University
4 PASTEUR - Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640)

Exposure to widely used inert fibrous nanomaterials (for example, glass fibres or carbon nanotubes) may result in asbestos-like lung pathologies, becoming an important environmental and health concern. However, the origin of the pathogenesis of such fibres has not yet been clearly established. Here we report an electrochemical nanosensor that is used to monitor and quantitatively characterize the flux and dynamics of reactive species release during the frustrated phagocytosis of glass nanofibres by single macrophages. We show the existence of an intense prolonged release of reactive oxygen and nitrogen species by single macrophages near their phagocytic cups. This continued massive leakage of reactive oxygen and nitrogen species damages peripheral cells and eventually translates into chronic inflammation and lung injury, as seen during in vitro co-culture and in vivo experiments

Mots clés: frustrated phagocytosis; glass fiber; nano-electrochemical sensor; ROS/RNS leakage; lung injury

Domaines: Toxicologie; Immunité innée; Pneumologie et système respiratoire

Yu-Ting Qi, Fu-Li Zhang, Si-Yu Tian, Hui-Qian Wu, Yi Zhao, et al.. Nanosensor detection of reactive oxygen and nitrogen species leakage in frustrated phagocytosis of nanofibres. Nature Nanotechnology, In press, ⟨10.1038/s41565-023-01575-0⟩. ⟨hal-04536074⟩