Rapid and specific detection of thiabendazole: enzymatic digestion-enabled fluorescent aptasensor

Mohamed Amine Berkal (1) , Jean-Jacques Toulme (2) , Corinne Nardin (1)

1 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux
2 Novaptech, Bordeaux 33076, France

Thiabendazole, a widely used broad-spectrum fungicide in agriculture, poses risks to human health. To monitor its presence in water, we propose a fluorescent aptasensor utilizing Escherichia coli exonuclease I (Exo I). The findings demonstrate a linear correlation between thiabendazole concentrations and digestion percentage, with a detection limit (LOD) exceeding 1 µM and a determination coefficient (R2) of 0.959. This aptamer-based fluorescence spectroscopy detection system holds promise for a rapid, specific, and sensitive analysis of thiabendazole in environmental waters and food matrices.

Mots clés: Biosensing; Enzymatic digestion; Fluorescence spectroscopy; Oligonucleotide switching structures; Thiabendazole

Domaines: Chimie; Polymères

Mohamed Amine Berkal, Jean-Jacques Toulme, Corinne Nardin. Rapid and specific detection of thiabendazole: enzymatic digestion-enabled fluorescent aptasensor. Analytical and Bioanalytical Chemistry, In press, ⟨10.1007/s00216-024-05309-6⟩. ⟨hal-04573964⟩