The absence of OprF increased biofilm formation through variation of the c- di-GMP level in Pseudomonas aeruginosa

Emeline Bouffartigues (1) , Joana A Moscoso (2) , Rachel Duchesne (1) , Thibaut Rosay (1) , Laurène Fito-Boncompte (1) , Gwendoline Gicquel (1) , Olivier Maillot (1) , Magalie Bénard (3) , Alexis Bazire (4) , Gerald Brenner-Weiss (5) , Olivier Lesouhaitier (1) , Patrice P. Lerouge (6) , Alain Dufour (4) , Nicole Orange (1) , Marc Feuilloley (7) , Joerg Overhage (5) , Alain Filloux (2) , Sylvie Chevalier (8)

1 LMSM - Laboratoire de Microbiologie Signaux et Microenvironnement
2 Department of Life Sciences
3 PRIMACEN - Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie
4 LBCM - Laboratoire de Biotechnologie et Chimie Marines
5 KIT - Karlsruhe Institute of Technology
6 Glyco-MEV - Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale
7 FAI - Université Le Havre Normandie - Faculté des Affaires Internationales
8 Chercheur indépendant

Introduction Pseudomonas aeruginosa is well known to be a human opportunistic pathogen able to adapt to many environmental conditions. The first cellular structure in contact with the environment is the outer membrane. In Pseudomonas genus, OprF is the major outer membrane protein. It is an aspecific porin allowing the diffusion of several types of small polar molecules. OprF is also a pleiotropic protein involved in other numerous cellular functions, as adhesion on biotic or abiotic surfaces, cell wall resistance and virulence of P. aeruginosa. Through a multiphenotypic study, we show here that the lack of OprF increased biofilm formation through modulation of the c-di-GMP level.

Domaines: Bactériologie

Emeline Bouffartigues, Joana A Moscoso, Rachel Duchesne, Thibaut Rosay, Laurène Fito-Boncompte, et al.. The absence of OprF increased biofilm formation through variation of the c- di-GMP level in Pseudomonas aeruginosa. Pseudomonas Conference, 2017, Washington, United States. ⟨hal-02366394⟩